MitoCheck is a database built off of RNA interference (RNAi) screens to identify all proteins that are required for mitosis in human cells. It includes:
-affinity purification and mass spectrometry to identify protein complexes and mitosis-specific phosphorylation sites on these
-small molecule inhibitors to determine which protein kinase is required for the phosphorylation of which substrate.
MitoCheck is also establishing clinical assays to validate mitotic proteins as prognostic biomarkers for cancer therapy.